Gemara

If some of the bloods of the bull and goat became mixed (in a third bucket before they should be mixed), surely the Haza'os (for Dam ha'Par or Dam ha'Sa'ir alone) are done from the buckets that were not mixed;

Question: Do we pour the mixed blood on the Yesod like Shirayim, or is it Nidcheh and spilled into the Amah?

Answer #1 (Rav Papa): Even according to the opinion that one bucket makes another Shirayim (if all Matanos Dam were done from one bucket, also Dam in the other bucket is considered Shirayim and is poured on the Yesod) says so only when Matanos could have been done from either bucket. Here, since we could not do Haza'ah from the mixed blood, it does not become Shirayim.

Answer #2 (Rav Huna brei d'Rav Yehoshua): Just the contrary! Even according to the opinion that one bucket is Docheh another says so only when the person was overtly Docheh (he could have given Matanos from the other, but chose not to). Here he was not overtly Docheh (he could not give from the mixed blood), so it is not Dachuy.

Note: Rashi's text says that the opinion that one bucket is Docheh a second says so only when none of the first bucket was put into the second. Here, some of the first bucket was put into the second, therefore there is no Dichuy.

(The following Tana'im argue about whether one bucket makes another Shirayim or is Docheh it.)

(Beraisa): It says "V'Es Damo Yishpoch" and "V'Es Kol Damah Yishpoch";

Question: If Kabalas Dam Chatas was done in four buckets, and blood from each of them was put on a different Keren of the Mizbe'ach, what is the source that the remaining blood in each bucket is poured on the Yesod?

Answer: We learn from "V'Es Kol Damah Yishpoch".

Suggestion: If Kabalah was done in four buckets, and blood from one of them was put on all the Keranos, perhaps all the remaining blood is poured on the Yesod!

Rejection: "V'Es Damo Yishpoch" (not all the blood) - the Shirayim from that bucket (from which blood was put) are poured on the Yesod, the others are poured into the Amah.

R. Elazar b'Rebbi Shimon says, "V'Es Kol Damah Yishpoch" teaches that (even) in this case all the Shirayim are poured on the Yesod.

Question: What does he learn from "V'Es Damo Yishpoch"?

Answer (Rav Ashi): This excludes blood left in the animal's neck (it is not poured on the Yesod).

Temurah 22b - Question (Rav Papa): What is the law of Avudah b'Kos (a lost Chatas was found after Shechitah and Kabalah of its replacement, and the blood was in a bucket)?

Version #1: He does not ask according to Rebbi, who says that a Chatas found after Hafrashah must die. Rather, he asks according to Chachamim, who say that a Chatas found after Hafrashah (before Hakravah) is Ro'eh. Perhaps after Kabalah, it is as if Zerikah was done (and therefore, it must die);

Or, perhaps as long as Zerikah was not done, it is Ro'eh!

Version #2: He asks (even) according to Rebbi. The case is, Kabalah was done in two buckets, and one of them was lost.

He does not ask according to the opinion that one bucket is Docheh another. All the more so, one bucket is Docheh a lost bucket!

He asks according to the opinion that one bucket makes another bucket Shirayim.

Perhaps this is only when both buckets are here, for Matanos could be made from either, but if one is lost, it is Nidcheh. Or, perhaps we do not distinguish!

This question is not resolved.

Rishonim

Rashi (Temurah 22b DH Aliba): If any of the blood is Nidcheh, the Korban is Pasul.

Question (Rashash): It is not clear why Rashi says that the question is whether or not the Korban is Pasul. It appears that the question is what to do with the lost blood if it is found (like Shitah Mekubetzes 10 explains).

Chazon Ish (Temurah 35:22 DH Ki): In Yoma, it is clear to Rav Papa that if we cannot give Matanos from a bucket it is Nidcheh. We must say that the second version in Temurah argues (and says that Rav Papa was unsure about this).

Rambam (Hilchos Pesulei ha'Mukdashim 2:21): If Kabalas Dam Chatas was done in four buckets, and blood from each of them was put on a different Keren, the remaining blood in all of them is poured on the Yesod. We learn from "V'Es Kol Damah Yishpoch".

If Kabalah was in four buckets, and blood from one of them was put on all the Keranos, Shirayim from that bucket are poured on the Yesod, the others are poured into the Amah.

Me'iri (57b DH ha'Dam): Only blood that flowed out and was collected in a Kli Shares can become Shirayim. Blood left in the neck is not Dam ha'Nefesh, so it is spilled to the Amah.

Acharonim

Aruch ha'Shulchan ha'Asid (128:10): The same applies to other Korbanos. If Kabalah for a Korban requiring two Matanos (that are like four) was done in two buckets, and blood from each was put on a different corner, the remaining blood in both is poured on the Yesod. If blood from one was put on both corners, Shirayim from it are poured on the Yesod, and the other is poured into the Amah. If a Korban requires only one Matanah, blood of one bucket is poured on the Mizbe'ach and the others are poured into the Amah.